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| Cell Line: | PC-3 | 
| Cell Type: | epithelial | 
| Description: | Human prostate carcinoma cells | 
| other names: | PC3, PC 3 | 
| application: | siRNA | 
| Recommendation: | ScreenFect siRNA | 
Recommendation based on the characteristics of ScreenFect transfection reagents.
Recommendation: ScreenFect®siRNA
Transfection conditions*
| Format: | 96-well | 24-well | 6-well | 
| ScreenFect volume: | 0,25 µl | 1 µl | 4 µl | 
| Amount of nucleic acid: | 1 pmol | 5 pmol | 25 pmol | 
| Cell number (cells/well) | - | - | - | 
| to achieve 90-100% confluency 48h post transfection | |||
| * The values given refer to the recommended One-Step method. See our manual for further details: Download Manual | |||
| Gene silencing efficiency: | - | - | - | 
| Viability: | - | - | - | 
| ScreenFect Enhacer addition leads to the following results: | |||
| Transefction efficiency increase: | |||
* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual
 
Alternative product: ScreenFect®A
| Format: | 96-well | 24-well | 6-well | 
| ScreenFect volume: | 0,3 µl | 1,8 µl | 6 µl | 
| Amount of nucleic acid: | 1 pmol | 5 pmol | 25 pmol | 
| Cell number (cells/well) | - | - | - | 
| to achieve 90-100% confluency 48h post transfection | |||
| * The values given refer to the recommended One-Step method. See our manual for further details: Download Manual | |||
| Gene silencing efficiency: | - | - | - | 
| Viability: | - | - | - | 
| ScreenFect Enhacer addition leads to the following results: | |||
| Transefction efficiency increase: | |||
* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual
 
 
