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| Cell Line: | NCI-H1299 |
| Cell Type: | epithelial lung cell |
| Description: | Human lung carcinoma |
| other names: | H1299, CRL-5803 |
| application: | pDNA |
| Recommendation: | ScreenFect A |
Recommendation based on in-house testing or customer feedback.
Recommendation: ScreenFect®A
Transfection conditions*
| Format: | 96-well | 24-well | 6-well |
| ScreenFect volume: | 0,375 µl | 2,1 µl | 6 µl |
| Amount of nucleic acid: | 75 ng | 300 ng | 1000 ng |
| DNA:reagent ratio (µg:µl): | 1:5 | 1:7 | 1:6 |
| Cell number (cells/well) | 20.000-25.000 | - | - |
| to achieve 90-100% confluency 24h post transfection | |||
| * The values given refer to the recommended One-Step method. See our manual for further details: Download Manual |
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| Transfection efficiency: | 77 % | - | - |
| Viability: | 92% | - | - |
| ScreenFect Enhancer addition leads to the following results: | |||
| Transfection efficiency increase: | 7% | - | - |
Note: The Two-Step method leads to slightly decreased transfection results. [96-well Format --> DNA:reagent ratio (µg:µl) 1:4, Transfection performance can be increased by 20% using ScreenFect Enhancer for 96 well format.
Alternative product: ScreenFect®A plus
| Format: | 96-well | 24-well | 6-well |
| ScreenFect volume: | 0,225µl | 1,2 µl | 4 µl |
| Amount of nucleic acid: | 75 ng | 300 ng | 1000 ng |
| DNA:reagent ratio (µg:µl): | 1:3 | 1:4 | 1:4 |
| Cell number (cells/well) | 20.000-25.000 | - | - |
| to achieve 90-100% confluency 24h post transfection | |||
| * The values given refer to the recommended One-Step method. See our manual for further details: Download Manual |
|||
| Transfection efficiency: | 76 % | - | - |
| Viability: | 90% | - | - |
| ScreenFect Enhacer addition leads to the following results: | |||
| Transefction efficiency increase: | 6% | - | - |
Note: The Two-Step method leads to slightly decreased transfection results. [96-well Format --> DNA:reagent ratio (µg:µl) 1:2, Transfection performance can be increased by 11% using ScreenFect Enhancer for 96 well format.
