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Cell Line: NCI-H1299
Cell Type: epithelial lung cell
Description:  Human lung carcinoma
other names: H1299, CRL-5803
application: pDNA
Recommendation:  ScreenFect A

Recommendation based on in-house testing or customer feedback.






Recommendation:  ScreenFect®A

Transfection conditions*


Format: 96-well 24-well 6-well
ScreenFect volume: 0,375 µl 2,1 µl 6 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:5 1:7 1:6
Cell number (cells/well) 20.000-25.000 - -
to achieve 90-100% confluency 24h post transfection
* The values given refer to the recommended One-Step method.
See our manual for further details: Download Manual
       
Transfection efficiency: 77 % - -
Viability: 92% - -
       
ScreenFect Enhancer addition leads to the following results:      
Transfection efficiency increase: 7% - -

Note: The Two-Step method leads to slightly decreased transfection results. [96-well Format --> DNA:reagent ratio (µg:µl) 1:4, Transfection performance can be increased by 20% using ScreenFect Enhancer for 96 well format.



ScreenFect_A-Aplus_neu_5.jpg




Alternative product:  ScreenFect®A plus


Format: 96-well 24-well 6-well
ScreenFect volume: 0,225µl 1,2 µl 4 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:3 1:4 1:4
Cell number (cells/well) 20.000-25.000 - -
to achieve 90-100% confluency 24h post transfection
* The values given refer to the recommended One-Step method.
See our manual for further details: Download Manual
       
Transfection efficiency: 76 % - -
Viability: 90% - -
       
ScreenFect Enhacer addition leads to the following results:      
Transefction efficiency increase: 6% - -

Note: The Two-Step method leads to slightly decreased transfection results. [96-well Format --> DNA:reagent ratio (µg:µl) 1:2, Transfection performance can be increased by 11% using ScreenFect Enhancer for 96 well format.



ScreenFect_A-Aplus_neu_4.jpg

ScreenFect GmbH • Hermann-von-Helmholtz-Platz 1 • D-76344 Eggenstein-Leopoldshafen • Tel: +49-721-608-29113 • Email: HXRze3Jdbn5veHhze3h+aTN+cnA@nospam

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