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Cell Line: PC-3
Cell Type: epithelial
Description:  Human prostate carcinoma cells 
other names: PC3, PC 3
application: pDNA
Recommendation:  ScreenFect A-plus

Recommendation based on in-house testing or customer feedback.






Recommendation:  ScreenFect®A plus

Transfection conditions*


Format: 96-well 24-well 6-well
ScreenFect volume: 0,225 µl 1,2 µl 4 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:3 1:4 1:4
Cell number (cells/well) 25.000 - 30.000 - -
to achieve 90-100% confluency 24h post transfection
* The values given refer to the recommended One-Step method. 
See our manual for further details: Download Manual   
       
Transfection efficiency: 45 % - -
Viability: 84% - -
       
ScreenFect Enhacer addition leads to the following results:   
Transfection efficiency increase: - - -
       
Note: The Two-Step method leads to slightly decreased transfection results.


ScreenFect_A-Aplus_neu_5.jpg




Alternative product:  ScreenFect®A


Format: 96-well 24-well 6-well
ScreenFect volume: 0,375 µl 2,1 µl 6 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:5 1:7 1:6
Cell number (cells/well) 25.000 - 30.000 - -
to achieve 90-100% confluency 24h post transfection      
* The values given refer to the recommended One-Step method.
See our manual for further details: Download Manual
       
Transfection efficiency: 44 % - -
Viability: 84% - -
       
ScreenFect Enhacer addition leads to the following results:   
Transfection efficiency increase: - - -
       
Note: The Two-Step method leads to slightly decreased transfection results.


ScreenFect_A-Aplus_neu_4.jpg

ScreenFect GmbH • Hermann-von-Helmholtz-Platz 1 • D-76344 Eggenstein-Leopoldshafen • Tel: +49-721-608-29113 • Email: Zg8IAAkmFQUUAwMIAAMFEkgFCQs@nospam

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