Suchergebnis für:
Cell Line: | PC-3 |
Cell Type: | epithelial |
Description: | Human prostate carcinoma cells |
other names: | PC3, PC 3 |
application: | pDNA |
Recommendation: | ScreenFect A-plus |
Recommendation based on in-house testing or customer feedback.
Recommendation: ScreenFect®A plus
Transfection conditions*
Format: | 96-well | 24-well | 6-well |
ScreenFect volume: | 0,225 µl | 1,2 µl | 4 µl |
Amount of nucleic acid: | 75 ng | 300 ng | 1000 ng |
DNA:reagent ratio (µg:µl): | 1:3 | 1:4 | 1:4 |
Cell number (cells/well) | 25.000 - 30.000 | - | - |
to achieve 90-100% confluency 24h post transfection | |||
* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual |
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Transfection efficiency: | 45 % | - | - |
Viability: | 84% | - | - |
ScreenFect Enhacer addition leads to the following results: | |||
Transfection efficiency increase: | - | - | - |
Note: The Two-Step method leads to slightly decreased transfection results. |
Alternative product: ScreenFect®A
Format: | 96-well | 24-well | 6-well |
ScreenFect volume: | 0,375 µl | 2,1 µl | 6 µl |
Amount of nucleic acid: | 75 ng | 300 ng | 1000 ng |
DNA:reagent ratio (µg:µl): | 1:5 | 1:7 | 1:6 |
Cell number (cells/well) | 25.000 - 30.000 | - | - |
to achieve 90-100% confluency 24h post transfection | |||
* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual |
|||
Transfection efficiency: | 44 % | - | - |
Viability: | 84% | - | - |
ScreenFect Enhacer addition leads to the following results: | |||
Transfection efficiency increase: | - | - | - |
Note: The Two-Step method leads to slightly decreased transfection results. |