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Cell Line: PANC-1
Cell Type: epithelial 
Description:  Human pancreas carcinoma
other names: PANC1
application: pDNA
Recommendation:  ScreenFect A

Recommendation based on in-house testing or customer feedback.






Recommendation:  ScreenFect®A

Transfection conditions*


Format: 96-well 24-well 6-well
ScreenFect volume: 0,225 - 0,3 µl 1,8 µl 6 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:3 - 1:4 1:6 1:6
Cell number (cells/well) 20.000-25.000 - -
to achieve 90-100% confluency 24h post transfection
* The values given refer to the recommended One-Step method.
See our manual for further details: Download Manual
       
Transfection efficiency: 47 % - -
Viability: 91 % - -
       
ScreenFect Enhacer addition leads to the following results:   
Transfection efficiency increase: - - -

Note: The Two-Step method leads to slightly decreased transfection results.



ScreenFect_A-Aplus_neu_5.jpg




Alternative product:  ScreenFect®A plus


Format: 96-well 24-well 6-well
ScreenFect volume: 0,15 µl 0,9 µl 4 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:2 1:3 1:4
Cell number (cells/well) 40.000 - -
* The values given refer to the recommended Two-Step method.
See our manual for further details: Download Manual
       
Transfection efficiency: 43 % - -
Viability: 87 % - -
       
ScreenFect Enhacer addition leads to the following results:      
Transfection efficiency increase: - - -

Note: The Two-Step method leads to slightly decreased transfection results.



ScreenFect_A-Aplus_neu_4.jpg

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