Suchergebnis für:
Cell Line: | PANC-1 |
Cell Type: | epithelial |
Description: | Human pancreas carcinoma |
other names: | PANC1 |
application: | pDNA |
Recommendation: | ScreenFect A |
Recommendation based on in-house testing or customer feedback.
Recommendation: ScreenFect®A
Transfection conditions*
Format: | 96-well | 24-well | 6-well |
ScreenFect volume: | 0,225 - 0,3 µl | 1,8 µl | 6 µl |
Amount of nucleic acid: | 75 ng | 300 ng | 1000 ng |
DNA:reagent ratio (µg:µl): | 1:3 - 1:4 | 1:6 | 1:6 |
Cell number (cells/well) | 20.000-25.000 | - | - |
to achieve 90-100% confluency 24h post transfection | |||
* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual |
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Transfection efficiency: | 47 % | - | - |
Viability: | 91 % | - | - |
ScreenFect Enhacer addition leads to the following results: | |||
Transfection efficiency increase: | - | - | - |
Note: The Two-Step method leads to slightly decreased transfection results.
Alternative product: ScreenFect®A plus
Format: | 96-well | 24-well | 6-well |
ScreenFect volume: | 0,15 µl | 0,9 µl | 4 µl |
Amount of nucleic acid: | 75 ng | 300 ng | 1000 ng |
DNA:reagent ratio (µg:µl): | 1:2 | 1:3 | 1:4 |
Cell number (cells/well) | 40.000 | - | - |
* The values given refer to the recommended Two-Step method. See our manual for further details: Download Manual |
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Transfection efficiency: | 43 % | - | - |
Viability: | 87 % | - | - |
ScreenFect Enhacer addition leads to the following results: | |||
Transfection efficiency increase: | - | - | - |
Note: The Two-Step method leads to slightly decreased transfection results.