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Cell Line: WT MEFs
Cell Type: fibroblast
Description:  mouse embryonic fibroblasts
other names: MEF
application: siRNA
Recommendation:  ScreenFect siRNA

Recommendation based on the characteristics of ScreenFect transfection reagents.





Recommendation:  ScreenFect®siRNA

Transfection conditions*


Format: 96-well 24-well 6-well
ScreenFect volume: 0,25 µl 1 µl 4 µl
Amount of nucleic acid: 1 pmol 5 pmol 25 pmol
       
Cell number (cells/well) - - -
to achieve 90-100% confluency 48h post transfection
* The values given refer to the recommended One-Step method.
See our manual for further details: Download Manual
       
Gene silencing efficiency: - - -
Viability: - - -
       
ScreenFect Enhacer addition leads to the following results:      
Transefction efficiency increase:      

* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual



ScreenFect_A-Aplus_neu_5.jpg




Alternative product:  ScreenFect®A


Format: 96-well 24-well 6-well
ScreenFect volume: 0,3 µl 1,8 µl 6 µl
Amount of nucleic acid: 1 pmol 5 pmol 25 pmol
       
Cell number (cells/well) - - -
to achieve 90-100% confluency 48h post transfection
* The values given refer to the recommended One-Step method.
See our manual for further details: Download Manual
       
Gene silencing efficiency: - - -
Viability: - - -
       
ScreenFect Enhacer addition leads to the following results:      
Transefction efficiency increase:      

* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual



ScreenFect_A-Aplus_neu_4.jpg

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