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Cell Line: | WT MEFs |
Cell Type: | fibroblast |
Description: | mouse embryonic fibroblasts |
other names: | MEF |
application: | siRNA |
Recommendation: | ScreenFect siRNA |
Recommendation based on the characteristics of ScreenFect transfection reagents.
Recommendation: ScreenFect®siRNA
Transfection conditions*
Format: | 96-well | 24-well | 6-well |
ScreenFect volume: | 0,25 µl | 1 µl | 4 µl |
Amount of nucleic acid: | 1 pmol | 5 pmol | 25 pmol |
Cell number (cells/well) | - | - | - |
to achieve 90-100% confluency 48h post transfection | |||
* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual |
|||
Gene silencing efficiency: | - | - | - |
Viability: | - | - | - |
ScreenFect Enhacer addition leads to the following results: | |||
Transefction efficiency increase: |
* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual
Alternative product: ScreenFect®A
Format: | 96-well | 24-well | 6-well |
ScreenFect volume: | 0,3 µl | 1,8 µl | 6 µl |
Amount of nucleic acid: | 1 pmol | 5 pmol | 25 pmol |
Cell number (cells/well) | - | - | - |
to achieve 90-100% confluency 48h post transfection | |||
* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual |
|||
Gene silencing efficiency: | - | - | - |
Viability: | - | - | - |
ScreenFect Enhacer addition leads to the following results: | |||
Transefction efficiency increase: |
* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual