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| Cell Line: | V79 |
| Cell Type: | fibroblast |
| Description: | Chinese hamster lung |
| other names: | V-79 |
| application: | pDNA |
| Recommendation: | ScreenFect A-plus |
Recommendation based on in-house testing or customer feedback.
Recommendation: ScreenFect®A plus
Transfection conditions*
| Format: | 96-well | 24-well | 6-well |
| ScreenFect volume: | 0,15 µl | 0,9 µl | 4 µl |
| Amount of nucleic acid: | 75 ng | 300 ng | 1000 ng |
| DNA:reagent ratio (µg:µl): | 1:2 | 1:3 | 1:4 |
| Cell number (cells/well) | 30.000-35.000 | - | - |
| to achieve 90-100% confluency 48h post transfection | |||
| * The values given refer to the recommended One-Step method. See our manual for further details: Download Manual |
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| Transfection efficiency: | 69 % | - | - |
| Viability: | 92% | - | - |
| ScreenFect Enhacer addition leads to the following results: | |||
| Transefection efficiency increase: | 13% | - | - |
Note: The Two-Step method leads to decreased transfection results.
Alternative product: ScreenFect®A
| Format: | 96-well | 24-well | 6-well |
| ScreenFect volume: | 0,3 µl | 1,8 µl | 6 µl |
| Amount of nucleic acid: | 75 ng | 300 ng | 1000 ng |
| DNA:reagent ratio (µg:µl): | 1:4 | 1:6 | 1:6 |
| Cell number (cells/well) | 30.000-35.000 | - | - |
| to achieve 90-100% confluency 48h post transfection | |||
| * The values given refer to the recommended One-Step method. See our manual for further details: Download Manual |
|||
| Transfection efficiency: | 53 % | - | - |
| Viability: | 94% | - | - |
| ScreenFect Enhacer addition leads to the following results: | |||
| Transfection efficiency increase: | 19% | - | - |
Note: The Two-Step method leads to decreased transfection results.
