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| Cell Line: | NIH3T3 |
| Cell Type: | fibroblast |
| Description: | Murine embryonic fibroblasts |
| other names: | NIH-3T3, 3T3, NIH/3T3 |
| application: | pDNA |
| Recommendation: | ScreenFect A |
Recommendation based on in-house testing or customer feedback.
Recommendation: ScreenFect®A
Transfection conditions*
| Format: | 96-well | 24-well | 6-well |
| ScreenFect volume: | 0,225 µl | 1,8 µl | 6 µl |
| Amount of nucleic acid: | 75 ng | 300 ng | 1000 ng |
| DNA:reagent ratio (µg:µl): | 1:3 | 1:6 | 1:6 |
| Cell number (cells/well) | 20.000-25.000 | - | - |
| to achieve 90-100% confluency 24h post transfection | |||
| * The values given refer to the recommended Two-Step method. See our manual for further details: Download Manual |
|||
| Transfection efficiency: | 44% | - | - |
| Viability: | 97% | - | - |
| ScreenFect Enhacer addition leads to the following results: | |||
| Transefection efficiency increase: | 12% | - | - |
Note: The One-Step method leads to slightly decreased transfection results.
Alternative product: ScreenFect®A plus
| Format: | 96-well | 24-well | 6-well |
| ScreenFect volume: | 0,15 µl | 0,9 µl | 4 µl |
| Amount of nucleic acid: | 75 ng | 300 ng | 1000 ng |
| DNA:reagent ratio (µg:µl): | 1:2 | 1:3 | 1:4 |
| Cell number (cells/well) | 20.000-25.000 | - | - |
| to achieve 90-100% confluency 24h post transfection | |||
| * The values given refer to the recommended Two-Step method. See our manual for further details: Download Manual |
|||
| Transfection efficiency: | 43% | - | - |
| Viability: | 97% | - | - |
| ScreenFect Enhacer addition leads to the following results: | |||
| Transfection efficiency increase: | 12% | - | - |
Note: The One-Step method leads to slightly decreased transfection results.
