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Cell Line: NIH3T3
Cell Type: fibroblast
Description:  Murine embryonic fibroblasts
other names: NIH-3T3, 3T3, NIH/3T3
application: pDNA
Recommendation:  ScreenFect A

Recommendation based on in-house testing or customer feedback.






Recommendation:  ScreenFect®A

Transfection conditions*


Format: 96-well 24-well 6-well
ScreenFect volume: 0,225 µl 1,8 µl 6 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:3 1:6 1:6
Cell number (cells/well) 20.000-25.000 - -
to achieve 90-100% confluency 24h post transfection
* The values given refer to the recommended Two-Step method.
See our manual for further details: Download Manual
       
Transfection efficiency: 44% - -
Viability: 97% - -
       
ScreenFect Enhacer addition leads to the following results:      
Transefection efficiency increase: 12% - -

Note: The One-Step method leads to slightly decreased transfection results.



ScreenFect_A-Aplus_neu_5.jpg




Alternative product:  ScreenFect®A plus


Format: 96-well 24-well 6-well
ScreenFect volume: 0,15 µl 0,9 µl 4 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:2 1:3 1:4
Cell number (cells/well) 20.000-25.000 - -
to achieve 90-100% confluency 24h post transfection
* The values given refer to the recommended Two-Step method.
See our manual for further details: Download Manual
       
Transfection efficiency: 43% - -
Viability: 97% - -
       
ScreenFect Enhacer addition leads to the following results:      
Transfection efficiency increase: 12% - -

Note: The One-Step method leads to slightly decreased transfection results.



ScreenFect_A-Aplus_neu_4.jpg

ScreenFect GmbH • Hermann-von-Helmholtz-Platz 1 • D-76344 Eggenstein-Leopoldshafen • Tel: +49-721-608-29113 • Email: waivp66BsqKzpKSvp6Site+irqw@nospam

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