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Cell Line: NIH3T3
Cell Type: fibroblast
Description:  Murine embryonic fibroblasts
other names: NIH-3T3, 3T3, NIH/3T3
application: co-transfection
Recommendation:  ScreenFect A

Recommendation based on the characteristics of ScreenFect transfection reagents.

Recommendation:  ScreenFect®A

Transfection conditions*

Format: 96-well 24-well 6-well
ScreenFect volume: 0,3 µl 1,8 µl 6 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:4 1:6 1:6
Cell number (cells/well) - - -
to achieve 90-100% confluency 24h post transfection
* The values given refer to the recommended One-Step method.
See our manual for further details: Download Manual
Transfection efficiency: - - -
Viability: - - -
ScreenFect Enhacer addition leads to the following results:      
Transefction efficiency increase:      

* The values given refer to the recommended One-Step method. 
See our manual for further details: Download Manual


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