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Cell Line: HepG2
Cell Type: Liver
Description:  Human hepatocarcinoma cells
other names: Hep G2
application: pDNA
Recommendation:  ScreenFect A

Recommendation based on in-house testing or customer feedback.






Recommendation:  ScreenFect®A

Transfection conditions*


Format: 96-well 24-well 6-well
ScreenFect volume: 0,3 µl 1,8 µl 6 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:4 1:6 1:6
Cell number (cells/well) 25.000-30.000 - -
to achieve 90-100% confluency 24h post transfection
* The values given refer to the recommended Two-Step method.
See our manual for further details: Download Manual
       
Transfection efficiency: 37 % - -
Viability: 95% - -
       
ScreenFect Enhacer addition leads to the following results:   
Transefction efficiency increase: 14% - -

Note: We recommend to coat the cell culture plates with Poly-L-Lysine for optimal cell growth.



ScreenFect_A-Aplus_neu_5.jpg




Alternative product:  ScreenFect®A plus


Format: 96-well 24-well 6-well
ScreenFect volume: 0,15 µl 0,9 µl 4 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:2 1:3 1:4
Cell number (cells/well) 25.000-30.000 - -
to achiev 90-100% confluency 24h post transfection
* The values given refer to the recommended Two-Step method.
See our manual for further details: Download Manual
       
Transfection efficiency: 32 % - -
Viability: 95% - -
       
ScreenFect Enhacer addition leads to the following results:   
Transfection efficiency increase: 6% - -

Note: We recommend to coat the cell culture plates with Poly-L-Lysine for optimal cell growth.



ScreenFect_A-Aplus_neu_4.jpg

ScreenFect GmbH • Hermann-von-Helmholtz-Platz 1 • D-76344 Eggenstein-Leopoldshafen • Tel: +49-721-608-29113 • Email: v9bR2dD-zNzN2trR2drcy5Hc0NI@nospam

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