Cell Line:	HepG2
Cell Type:	Liver
Description:	Human hepatocarcinoma cells
other names:	Hep G2
application:	pDNA
Recommendation:	ScreenFect A

Recommendation based on in-house testing or customer feedback.

Recommendation: ScreenFect®A

Transfection conditions*

Format:	96-well	24-well	6-well
ScreenFect volume:	0,3 µl	1,8 µl	6 µl
Amount of nucleic acid:	75 ng	300 ng	1000 ng
DNA:reagent ratio (µg:µl):	1:4	1:6	1:6
Cell number (cells/well)	25.000-30.000	-	-
to achieve 90-100% confluency 24h post transfection
* The values given refer to the recommended Two-Step method. See our manual for further details: Download Manual

Transfection efficiency:	37 %	-	-
Viability:	95%	-	-

ScreenFect Enhacer addition leads to the following results:
Transefction efficiency increase:	14%	-	-

Note: We recommend to coat the cell culture plates with Poly-L-Lysine for optimal cell growth.

Alternative product: ScreenFect®A plus

Format:	96-well	24-well	6-well
ScreenFect volume:	0,15 µl	0,9 µl	4 µl
Amount of nucleic acid:	75 ng	300 ng	1000 ng
DNA:reagent ratio (µg:µl):	1:2	1:3	1:4
Cell number (cells/well)	25.000-30.000	-	-
to achiev 90-100% confluency 24h post transfection
* The values given refer to the recommended Two-Step method. See our manual for further details: Download Manual

Transfection efficiency:	32 %	-	-
Viability:	95%	-	-

ScreenFect Enhacer addition leads to the following results:
Transfection efficiency increase:	6%	-	-

Note: We recommend to coat the cell culture plates with Poly-L-Lysine for optimal cell growth.