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| Cell Line: | HeLa |
| Cell Type: | Epithelial cells |
| Description: | Human cervix epitheloid carcinoma |
| other names: | HeLa |
| application: | pDNA |
| Recommendation: | ScreenFect A |
Recommendation based on in-house testing or customer feedback.
Recommendation: ScreenFect®A
Transfection conditions*
| Format: | 96-well | 24-well | 6-well |
| ScreenFect volume: | 0,375 µl | 1,2 µl | 6 µl |
| Amount of nucleic acid: | 75 ng | 300 ng | 1000 ng |
| DNA:reagent ratio (µg:µl): | 1:5 | 1:4 | 1:6 |
| Cell number (cells/well) | 30.000 - 35.000 | 150.000 | 400.000 |
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to achieve 90-100% confluency 24h post transfection |
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| * The values given refer to the recommended One-Step method. See our manual for further details: Download Manual |
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| Transfection efficiency: | 78 % | 85 % | 82 % |
| Viability: | 87 % | 83 % | 86 % |
| ScreenFect Enhacer addition leads to the following results: | |||
| Transfection efficiency increase: | - | - | - |
Note: The Two-Step method leads to slightly decreased transfection results.
Alternative product: ScreenFect®A plus
| Format: | 96-well | 24-well | 6-well |
| ScreenFect volume: | 0,15 µl | 0,9 µl | 4 µl |
| Amount of nucleic acid: | 75 ng | 300 ng | 1000 ng |
| DNA:reagent ratio (µg:µl): | 1:2 | 1:3 | 1:4 |
| Cell number (cells/well) | 30.000 - 35.0000 | 150.000 | 400.000 |
| * The values given refer to the recommended One-Step method. See our manual for further details: Download Manual |
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| Transfection efficiency: | 76 % | 81% | 80% |
| Viability: | 84% | 74% | 74% |
| ScreenFect Enhacer addition leads to the following results: | |||
| Transfection efficiency increase: | - | - | - |
* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual
