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Cell Line: HeLa
Cell Type: Epithelial cells
Description:  Human cervix epitheloid carcinoma
other names: HeLa
application: pDNA
Recommendation:  ScreenFect A

Recommendation based on in-house testing or customer feedback.






Recommendation:  ScreenFect®A

Transfection conditions*


Format: 96-well 24-well 6-well
ScreenFect volume: 0,375 µl 1,2 µl 6 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:5 1:4 1:6
Cell number (cells/well) 30.000 - 35.000 150.000 400.000

to achieve 90-100% confluency 24h post transfection

* The values given refer to the recommended One-Step method.
See our manual for further details: Download Manual
       
Transfection efficiency: 78 % 85 % 82 %
Viability: 87 % 83 % 86 %
       
ScreenFect Enhacer addition leads to the following results:      
Transfection efficiency increase: - - -

Note: The Two-Step method leads to slightly decreased transfection results.



ScreenFect_A-Aplus_neu_5.jpg




Alternative product:  ScreenFect®A plus


Format: 96-well 24-well 6-well
ScreenFect volume: 0,15 µl 0,9 µl 4 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:2 1:3 1:4
Cell number (cells/well) 30.000 - 35.0000 150.000 400.000
* The values given refer to the recommended One-Step method.
See our manual for further details: Download Manual
       
Transfection efficiency: 76 % 81% 80%
Viability: 84% 74% 74%
       
ScreenFect Enhacer addition leads to the following results:      
Transfection efficiency increase: - - -

* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual



ScreenFect_A-Aplus_neu_4.jpg

ScreenFect GmbH • Hermann-von-Helmholtz-Platz 1 • D-76344 Eggenstein-Leopoldshafen • Tel: +49-721-608-29113 • Email: xK2qoquEt6e2oaGqoqGnsOqnq6k@nospam

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