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Cell Line: HeLa
Cell Type: Epithelial cells
Description:  Human cervix epitheloid carcinoma
other names: HeLa
application: co-transfection
Recommendation:  ScreenFect A

Recommendation based on the characteristics of ScreenFect transfection reagents.






Recommendation:  ScreenFect®A

Transfection conditions*


Format: 96-well 24-well 6-well
ScreenFect volume: 0,3 µl 2 µl 6 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:4 1:7 1:6
Cell number (cells/well) 45.000 220.000 500.000
to achieve 90-100% confluency 24h post transfection
* The values given refer to the recommended One-Step method.
See our manual for further details: Download Manual
       
Transfection efficiency: - - -
Viability: - - -
       
ScreenFect Enhacer addition leads to the following results:      
Transefction efficiency increase:      

* The values given refer to the recommended One-Step method.
See our manual for further details: Download Manual



ScreenFect_A-Aplus_neu_5.jpg




ScreenFect GmbH • Hermann-von-Helmholtz-Platz 1 • D-76344 Eggenstein-Leopoldshafen • Tel: +49-721-608-29113 • Email: bAUCCgMsHw8eCQkCCgkPGEIPAwE@nospam

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