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Cell Line: HEK293
Cell Type: Epithelial cells
Description:  Human embryonic kidney
other names: HEK-293, 293
application: pDNA
Recommendation:  ScreenFect A-plus

Recommendation based on in-house testing or customer feedback.






Recommendation:  ScreenFect®A-plus

Transfection conditions*


Format: 96-well 24-well 6-well
ScreenFect volume: 0,15 µl 0,9 µl 3 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:2 1:3 1:3
Cell number (cells/well) 45.000 220.000 500.000
* The values given refer to the recommended One-Step method.
See our manual for further details: Download Manual
       
Transfection efficiency: 84 % 95 % 92 %
Viability: 92 % 94 % 87 %
       
ScreenFect Enhacer addition leads to the following results:      
Transfection efficiency increase: - - -

Note: The Two-Step method leads to slightly decreased transfection results.



ScreenFect_A-Aplus_neu_5.jpg




Alternative product:  ScreenFect®A


Format: 96-well 24-well 6-well
ScreenFect volume: 0,375 µl 1 µl 4 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:5 1:4 1:5
Cell number (cells/well) 45.000 220.000 500.000
* The values given refer to the recommended One-Step method.
See our manual for further details: Download Manual
       
Transfection efficiency: 80 % 94 % 93 %
Viability: 92 % 95 % 91 %
       
ScreenFect Enhacer addition leads to the following results:      
Transfection efficiency increase: - - -

* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual



ScreenFect_A-Aplus_neu_4.jpg

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