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| Cell Line: | HEK293 |
| Cell Type: | Epithelial cells |
| Description: | Human embryonic kidney |
| other names: | HEK-293, 293 |
| application: | pDNA |
| Recommendation: | ScreenFect A-plus |
Recommendation based on in-house testing or customer feedback.
Recommendation: ScreenFect®A-plus
Transfection conditions*
| Format: | 96-well | 24-well | 6-well |
| ScreenFect volume: | 0,15 µl | 0,9 µl | 3 µl |
| Amount of nucleic acid: | 75 ng | 300 ng | 1000 ng |
| DNA:reagent ratio (µg:µl): | 1:2 | 1:3 | 1:3 |
| Cell number (cells/well) | 45.000 | 220.000 | 500.000 |
| * The values given refer to the recommended One-Step method. See our manual for further details: Download Manual |
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| Transfection efficiency: | 84 % | 95 % | 92 % |
| Viability: | 92 % | 94 % | 87 % |
| ScreenFect Enhacer addition leads to the following results: | |||
| Transfection efficiency increase: | - | - | - |
Note: The Two-Step method leads to slightly decreased transfection results.
Alternative product: ScreenFect®A
| Format: | 96-well | 24-well | 6-well |
| ScreenFect volume: | 0,375 µl | 1 µl | 4 µl |
| Amount of nucleic acid: | 75 ng | 300 ng | 1000 ng |
| DNA:reagent ratio (µg:µl): | 1:5 | 1:4 | 1:5 |
| Cell number (cells/well) | 45.000 | 220.000 | 500.000 |
| * The values given refer to the recommended One-Step method. See our manual for further details: Download Manual |
|||
| Transfection efficiency: | 80 % | 94 % | 93 % |
| Viability: | 92 % | 95 % | 91 % |
| ScreenFect Enhacer addition leads to the following results: | |||
| Transfection efficiency increase: | - | - | - |
* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual
