Recommendation based on in-house testing or customer feedback.

Recommendation: ScreenFect®A-plus

Transfection conditions*

Format:	96-well	24-well	6-well
ScreenFect volume:	0,3 µl	1,2 µl	4 µl
Amount of nucleic acid:	75 ng	300 ng	1000 ng
DNA:reagent ratio (µg:µl):	1:4	1:4	1:4
Cell number (cells/well)	20.000	-	-
to achieve 90-100% confluency 24h post transfection
* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual

Transfection efficiency:	78 %	-	-
Viability:	93%	-	-

ScreenFect Enhacer addition leads to the following results:
Transfection efficiency increase:	5%	-	-
Note: The Two-Step method leads to decreased transfection results. [96-well Format --> DNA:reagent ratio (µg:µl) 1:3, the addition of ScreenFect Enhancer increase 16% the transfection efficiency.]

* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual

Alternative product: ScreenFect®A

Format:	96-well	24-well	6-well
ScreenFect volume:	0,3 µl	1,8 µl	6 µl
Amount of nucleic acid:	75 ng	300 ng	1000 ng
DNA:reagent ratio (µg:µl):	1:4	1:6	1:6
Cell number (cells/well)	20.000	-	-
to achiev 90-100% confluency 24h post transfection
* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual

Trensfection efficiency:	77 %	-	-
Viability:	95%	-	-

ScreenFect Enhacer addition leads to the following results:
Transfection efficiency increase:	6%	-	-
Note: The Two-Step method leads to decreased transfection results. [96-well Format --> DNA:reagent ratio (µg:µl) 1:5, the addition of ScreenFect Enhancer increase 25% the transfection efficiency.]

* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual