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Cell Line: C2C12
Cell Type: Myoblast
Description:  Murine myoblasts 
other names: C2C12
application: pDNA
Recommendation:  ScreenFect A-plus

Recommendation based on in-house testing or customer feedback.






Recommendation:  ScreenFect®A-plus

Transfection conditions*


Format: 96-well 24-well 6-well
ScreenFect volume: 0,3 µl 1,2 µl 4 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:4 1:4 1:4
Cell number (cells/well) 20.000 - -
to achieve 90-100% confluency 24h post transfection
* The values given refer to the recommended One-Step method.
See our manual for further details: Download Manual
       
Transfection efficiency: 78 % - -
Viability: 93% - -
       
ScreenFect Enhacer addition leads to the following results:      
Transfection efficiency increase: 5% - -
Note: The Two-Step method leads to decreased transfection results. [96-well Format --> DNA:reagent ratio (µg:µl) 1:3, the addition of ScreenFect Enhancer increase 16% the transfection efficiency.]
       

* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual



ScreenFect_A-Aplus_neu_5.jpg




Alternative product:  ScreenFect®A


Format: 96-well 24-well 6-well
ScreenFect volume: 0,3 µl 1,8 µl 6 µl
Amount of nucleic acid: 75 ng 300 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:4 1:6 1:6
Cell number (cells/well) 20.000 - -
to achiev 90-100% confluency 24h post transfection
* The values given refer to the recommended One-Step method.
See our manual for further details: Download Manual
       
Trensfection efficiency: 77 % - -
Viability: 95% - -
       
ScreenFect Enhacer addition leads to the following results:      
Transfection efficiency increase: 6% - -
Note: The Two-Step method leads to decreased transfection results. [96-well Format --> DNA:reagent ratio (µg:µl) 1:5, the addition of ScreenFect Enhancer increase 25% the transfection efficiency.]

* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual



ScreenFect_A-Aplus_neu_4.jpg

ScreenFect GmbH • Hermann-von-Helmholtz-Platz 1 • D-76344 Eggenstein-Leopoldshafen • Tel: +49-721-608-29113 • Email: Zg8IAAkmFQUUAwMIAAMFEkgFCQs@nospam

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