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Cell Line: WT MEFs
Cell Type: fibroblast
Description:  mouse embryonic fibroblasts
other names: MEF
application: pDNA
Recommendation:  ScreenFect A

Recommendation based on in-house testing or customer feedback.






Recommendation:  ScreenFect®A

Transfection conditions*


Format: 96-well 24-well 6-well
ScreenFect volume: 0,3 µl 2 µl 7 µl
Amount of nucleic acid: 75 ng 500 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:4 1:4 1:7
Cell number (cells/well) 30.000-35.000 100.000 300.000
to achieve 90-100% confluency 24h post transfection
* The values given refer to the recommended One-Step method.
See our manual for further details: Download Manual
       
Transfection efficiency: 58% 56% -
Viability: 81% 90% -
       
ScreenFect Enhacer addition leads to the following results:      
Transefection efficiency increase: 5,5% - -

Note: The Two-Step method leads to decreased transfection results.



ScreenFect_A-Aplus_neu_5.jpg




Alternative product:  ScreenFect®A plus


Format: 96-well 24-well 6-well
ScreenFect volume: 0,225 µl 1 µl 4 µl
Amount of nucleic acid: 75 ng 500 ng 1000 ng
DNA:reagent ratio (µg:µl):  1:3 1:2 1:4
Cell number (cells/well) 30.000-35.000 100.000 300.000
to achieve 90-100% confluency 24h post transfection
* The values given refer to the recommended One-Step method.
See our manual for further details: Download Manual
       
Transfection efficiency: - 51% -
Viability: - 78% -
       
ScreenFect Enhacer addition leads to the following results:      
Transfection efficiency increase: 12% - -

Note: The Two-Step method leads to decreased transfection results.



ScreenFect_A-Aplus_neu_4.jpg

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