Suchergebnis für:
| Cell Line: | HeLa |
| Cell Type: | Epithelial cells |
| Description: | Human cervix epitheloid carcinoma |
| other names: | HeLa |
| application: | siRNA |
| Recommendation: | ScreenFect siRNA |
Recommendation: ScreenFect®siRNA
Transfection conditions*
| Format: | 96-well | 24-well | 6-well |
| ScreenFect volume: | 0,25 µl | 1 µl | 4 µl |
| Amount of nucleic acid: | 1 pmol | 5 pmol | 25 pmol |
| Cell number (cells/well) | 25.000 | - | - |
| to achieve 90-100% confluency 48h post transfection | |||
| * The values given refer to the recommended One-Step method. See our manual for further details: Download Manual |
|||
| Gene silencing efficiency: | 91% | - | - |
| Viability: | 90% | - | - |
| 48h post transfection | |||
| ScreenFect Enhacer addition leads to the following results: | |||
| Transefction efficiency increase: | |||
* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual
Alternative product: ScreenFect®A
| Format: | 96-well | 24-well | 6-well |
| ScreenFect volume: | 0,35 µl | 1 µl | 4 µl |
| Amount of Nucleic acid: | 1 pmol | 5 pmol | 25 pmol |
| Cell number (cells/well) | 25.000 | - | - |
| to achieve 90-100% confluency 48h post transfection | |||
| * The values given refer to the recommended One-Step method. See our manual for further details: Download Manual |
|||
| Gene silencing efficiency: | 63% | - | - |
| Viability: | 95% | - | - |
| 48h post transfection | |||
| ScreenFect Enhacer addition leads to the following results: | |||
| Transefction efficiency increase: | |||
* The values given refer to the recommended One-Step method. See our manual for further details: Download Manual
