Scientists from the Hokkaido University, Sapporo, Japan successfully transfected OLHNI-2 cells, a medaka fin-derived cell line, with ScreenFect A, which they used in their study of the role for melato.
We are happy to let you know, that we start our sales activities with BocaScientific as our new Sales Partner in the US.
We are happy to announce that our reagent ScreenFect®A works great for CRISPR/Cas9 transfection!
miRNA dysregulation has recently been linked to human obesity and its related complications such as type 2 diabetes.
Several LIM domain proteins regulate transcription. They are thought to act through their LIM protein-protein interaction domains as adaptors for the recruitment of transcriptional co-regulators. An intriguing example is nTRIP6, the nuclear isoform of the focal adhesion protein TRIP6. nTRIP6 interacts with AP-1 and enhances its transcriptional activity. nTRIP6 is also essential for the transrepression of AP-1 by the glucocorticoid receptor (GR), by mediating GR tethering to promoter- bound AP-1.
Low-density lipoprotein receptor-related proteins 5 and 6 (LRP5/6) function as transmembrane receptors to transduce Wnt signals. A key mechanism for signalling is Wnt-induced serine/threonine phosphorylation at conserved PPPSPxS motifs in the LRP6 cytoplas-mic domain, which promotes pathway activation. Conserved tyro-sine residues are positioned close to all PPPSPxS motifs, which suggests they have a functional significance. Using a cell culture-based cDNA expression screen, we identified the non-receptor tyro-sine kinases Src and Fer as novel LRP6 modifiers.
Background: Long non-coding RNAs (lncRNA) play an important role in carcinogenesis; knowledge on lncRNA expression in renal cell carcinoma is rudimental. As a basis for biomarker development, we aimed to explore the lncRNA expression profile in clear cell renal cell carcinoma (ccRCC) tissue.
Gene delivery is the transfer of nucleic acid molecules (DNA or RNA) into cells, a process commonly termed cell transfection. If a cell is transfected with a plasmid DNA molecule harboring a gene of interest, that gene will be over-express, resulting in an increase of the gene product within the cell, thus allowing the nature of its activity to be analyzed. In addition to the “gain-of-function” transfection method, “loss-of-function” methods can likewise be achieved by transfection of nucleic acid molecules called short interfering RNA (siRNA). Mechanistically, siRNA transfection results in the silencing of endogenous gene transcripts (mRNA) complementary to the siRNA molecule transfected.
Incella’s technology: our thiol-yne based combinatorial click chemistry method allows parallel, high-throughput synthesis of hundreds of novel lipid-like chemicals in a cost effective manner. Every lipid-like molecule can be used to produce a novel transfection reagent with different properties. We combine our expertise in chemistry and biology to create an interdisciplinary research and development environment that encompasses chemical design, synthesis, liposomal reagent preparation, cell-based screening assays as well as identification and final optimization of novel transfection reagents.